Analyzing flow cytometry data using FlowJo

software
live training

Analyzing flow cytometry data using FlowJo

Target Audience:
All VIB staff
Location:

Ghent

General context

FlowJo version10 is a newly designed version of the famous FlowJo software. In contrast with previous versions (iOS only), version 10 is now a multi-platform software tool (Windows, iOS, Linux). It now has a very intuitive user interface designed to analyze your data in a very practical and fast way.
The training will be focused on getting familiar with all the tools available within the software.

Learning outcomes

Learn researchers how to analyze flow cytometry data in an efficient way using FlowJo v10,the world’s nr.1 analysis software tool for flow cytometry data.
This training will make you familiar with all the basic and advanced features of FlowJo v10. In the end you will be able to analyze and visualize your flow data and learn how to get the statistics out of it

Required skills

Prior knowledge in general flow cytometry is required but the general principles will be repeated.

Course materials

Trainers

Gert Van Isterdael
VIB Flow Core Ghent, BE

As head of the VIB Flow Core,  Gert provides flow cytometry expertise to the users of the core facility by giving technical help to the scientists, maintaining the machines, implicating high quality SOPs as well as providing both experimental setup and data analysis consultations. The Flow Core develops novel automated analysis techniques for multi-parameter flow cytometry data to remove user bias in close collaboration with the on-site Bioinformatics team headed by Prof. Yvan Saeys. 

Contact Gert Van Isterdael :

Program

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Flow cytometry, multicolor flow and compensation: general principles
General layout introduction
Workspace overview
Creating, handling and saving workspaces
Creation of groups
Gating procedures
Creating statistics tables with the Table editor
Data visualization with Layout editor
Batch analysis features
Automated compensation
Practical exercise with some datasets

Practical info

Location & Venue

04 November 2019

Ghent - Clemenspoort

Overwale 3
9000 Ghent
Belgium

Public transport

04 November 2019

Ghent - Clemenspoort
Public transport

The Clemenspoort, the Clemenshuis, and the Clemenshof are integrated into a network of other buildings on the grounds of the former Redemptorist monastery, now the Overwale street.

Located just 250 meters from Ghent St. Pieters Station.

Bike

04 November 2019

Ghent - Clemenspoort
Bike

40 bike parking lots available.

Route description

04 November 2019

Ghent - Clemenspoort
Parking

Parking facilities available (€ 1.80 per hour). GPS address: Sint-Denijslaan 251. Take the driveway of the HoGent Sports Hall (not the HoGent Campus) and drive all the way to the end - past the HoGent parking areas. If you are registered in the 411 app, activate your license plate under the option 'Garage parking via license plate'. The barrier will open automatically, and payment will be processed via 4411.

Venue contact

04 November 2019

Ghent - Clemenspoort
Location contact

+32 476 46 10 40

info@clemenspoort.be