Analyzing flow cytometry data using FlowJo
Analyzing flow cytometry data using FlowJo
18 November 2025, 9h30- 17h00
General context
Are you a researcher working with flow cytometry data?
This introductory FlowJo course is designed to help you get started with one of the most widely used analysis tools in the field. With the release of FlowJo Version 10—now available on Windows, macOS, and Linux—users benefit from a streamlined, intuitive interface that simplifies complex data analysis. This training will guide you through the core functionalities of the software, helping you build confidence in gating strategies, population analysis, and data visualization. By the end of the session, you’ll be equipped to independently analyze your own datasets and integrate FlowJo into your research workflow.
- Identify the main components of the FlowJo Version 10 interface and describe their functions.
- Import and organize flow cytometry data files within FlowJo for efficient analysis.
- Apply basic gating strategies to define and analyze cell populations.
- Interpret graphical outputs such as histograms and dot plots to conclude cytometry data.
- Demonstrate the ability to save, export, and document analysis results for reporting and collaboration.
Prior knowledge of general flow cytometry is required, but the general principles will be repeated.
Trainers
Gert Van Isterdael
As head of the VIB Flow Core, Gert provides flow cytometry expertise to the users of the core facility by giving technical help to the scientists, maintaining the machines, implicating high quality SOPs as well as providing both experimental setup and data analysis consultations. The Flow Core develops novel automated analysis techniques for multi-parameter flow cytometry data to remove user bias in close collaboration with the on-site Bioinformatics team headed by Prof. Yvan Saeys.
Program
- Flow cytometry, multicolor flow, and compensation: general principles
- General layout introduction
- Workspace overview
- Creating, handling, and saving workspaces
- Creation of groups
- Gating procedures
- Creating statistics tables with the Table editor
- Data visualization with Layout editor
- Batch analysis features
- Automated compensation
- Practical exercise with some datasets
Practical info
18 November 2025
Leuven - Campus Gasthuisberg
Herestraat 49
3000 Leuven
Belgium
18 November 2025
Leuven - Campus Gasthuisberg
Leuven train station is approximately 4 km from the campus Gasthuisberg. Buses depart very regularly to the Gasthuisberg Campus from Leuven train station.
For tickets and up to date schedules visit website De Lijn or use the De Lijn App.
Exit at the very last bus stop ‘Gasthuisberg Campus) make sure not to exit at ‘Gasthuisberg Kliniek =
hospital)
Once you get off the bus, go to your right and go up the street until you see stairs to your left. Climb the stairs all the way up between two buildings. Look for the signs containing blue circles and white numbers and follow them all the way from 2 up to number 12. You will cross several buildings and the walk will take max. 10 minutes. When you reach number 12, you have reached O&N 5, where you can find the reception.
18 November 2025
Leuven - Campus Gasthuisberg
The Gasthuisberg campus is west of the Leuven ring. Our buildings are situated behind the hospital (see black arrow near ‘RING NOORD’ on the map). If you come from the city center, you will arrive on campus from the east side (taking Mgr. Van Waeyenberglaan and roundabout Het Teken). If you come from Heverlee or Campus Arenberg, you will arrive from the west side (taking the Celestijnenlaan and Taborweg). When you arrive uphill at the intersection with the Rega Institute and the Red Cross building, you need to take the road down to the bicycle parking at Rega Institute (see map) where you can park. Next, follow the ‘Onderwijs & Navorsing blue route’ description below (start point is #9).
18 November 2025
Leuven - Campus Gasthuisberg
You arrive via the E314 highway – EXIT 16
After 200 m, keep left to drive underneath the roundabout and into the tunnel.
Keep left and follow the arrows to Parking West.
You arrive via the Leuven ring road
At the roundabout, take the exit towards ‘Spoed / Ziekenhuis’.
Follow the ring around Campus Gasthuisberg and pass the entrance of SPOED.
Continue right until you arrive at a second roundabout.
Follow the directions for E314.
Near the Fire Brigade Station, make a U-turn and follow the directions for ‘Ziekenhuis’.
Keep left and drive downhill, underneath the roundabout and into the tunnel.
Keep left and follow the arrows to Parking West.
Next, leave the parking at level +1 using the big stairs near the vending machines.
Follow the red carpet and arrows to 'Onco Dagcentrum' to get to the O&N buildings. Take the stairs on your left after exiting Parking West. You are now in front of the O&N4 building; on the left you will find O&N5.