FlowSOM for handling cytometry data

live training

FlowSOM for handling cytometry data

Target Audience:
VIB PhD Student
VIB Postdoc
VIB Staff Scientist
VIB Group leader & Expert
VIB Technical support
Location:

Ghent

Duration:

6 June 2023

General context

The number of markers, cells and samples measured in cytometry experiments keeps increasing. While this offers unique opportunities, it also complicates the traditional manual gating process, which becomes time-consuming and subjective. Replacing the manual gating by an automated pipeline can solve these issues. In this workshop, we will explore how to handle cytometry data in R, with a focus on the FlowSOM clustering algorithm.

Objectives

Participants will learn how to handle cytometry data in R, including quality control, visualization, clustering and comparison of samples. This will be demonstrated on an example dataset, but participants are welcome to bring some of their own fcs files to explore in addition.

Required skills

Prior experience with traditional manual cytometry analysis and R is strongly recommended. If you have no experience with R you can attend the R introduction training.

Trainers

Sofie Van Gassen
SaeysLab, VIB-UGent Center for Inflammation Research

Sofie is Postdoc at Ghent University working on optimization of the analysis of flow cytometry data by developing suitable machine learning algorithms. She's the creator of the FlowSOM algorithm. 

Contact Sofie Van Gassen :

Program

Schedule will be published later

Practical info

Location & Venue

06 June 2023

Ghent - VIB/UGent FSVM II

Technologiepark 75
9052 Zwijnaarde
Belgium

Public transport

06 June 2023

Ghent - VIB/UGent FSVM II
Public transport

From Ghent Sint-Pieters station, you can take bus 49, 50 or 70 to Technologiepark. Please check Routeplanner De Lijn for schedules.

Route description

06 June 2023

Ghent - VIB/UGent FSVM II
Parking

Route description

There is only one entrance to Technologiepark. At the entrance, please take a ticket and have it validated at the reception desk of the FSVM building. Parking will only be allowed in regular parking spots (for instance in front of the building) and in the new parking tower. Parking alongside the roads or in other places where there is no regular parking is prohibited and after initial warnings, fines will be issued (€ 50).

Venue contact

06 June 2023

Ghent - VIB/UGent FSVM II