Galaxy Training: Using Galaxy for RNA-seq

basic bioinformatics
ELIXIR
live training

Galaxy Training: Using Galaxy for RNA-seq

Target Audience:
All VIB staff
Location:

Ghent

General context

Galaxy software framework is an open-source application. It is a system that enables researchers without informatics expertise to perform computational biology analyses through the web.

Learning outcomes

This practical aims to familiarize you with the Galaxy user interface & execute a complete analysis of RNA-seq data.

It will teach you how to perform basic tasks such as importing data, running tools, working with histories, creating workflows, and sharing your work. In this tutorial we will introduce you to bare basics of Galaxy:

  • Getting data from external databases such as UCSC
  • Performing simple data manipulation
  • Understanding Galaxy’s History system
  • Creating and running a workflow

The RNA-seq data analysis part comprises:

  • Filtering and quality control of reads
  • Mapping of reads to reference genomes
  • Examples of downstream data-analysis possibilities (variant calling, differential expression, etc.) 

This workshop is organised by ELIXIR Belgium.

Event intended for

Biologists and other life science researchers who would like an introduction to Galaxy, its workflows and use it for NGS analysis.

Required skills

No prior bioinformatis knowledge is required.

Program

Working with Galaxy user interface

RNA-seq analysis with Galaxy

Practical info

Location & Venue

08 October 2019

Ghent - Clemenspoort

Overwale 3
9000 Ghent
Belgium

Public transport

08 October 2019

Ghent - Clemenspoort
Public transport

The Clemenspoort, the Clemenshuis, and the Clemenshof are integrated into a network of other buildings on the grounds of the former Redemptorist monastery, now the Overwale street.

Located just 250 meters from Ghent St. Pieters Station.

Bike

08 October 2019

Ghent - Clemenspoort
Bike

40 bike parking lots available.

Route description

08 October 2019

Ghent - Clemenspoort
Parking

Parking facilities available (€ 1.80 per hour). GPS address: Sint-Denijslaan 251. Take the driveway of the HoGent Sports Hall (not the HoGent Campus) and drive all the way to the end - past the HoGent parking areas. If you are registered in the 411 app, activate your license plate under the option 'Garage parking via license plate'. The barrier will open automatically, and payment will be processed via 4411.

Venue contact

08 October 2019

Ghent - Clemenspoort
Location contact

+32 476 46 10 40

info@clemenspoort.be