training

Liquid-Liquid Phase Separation

Liquid-Liquid Phase Separation

14 December 2020 - 15 December 2020

Online training

Liquid-Liquid Phase Separation

online
Location:

Online training

Start date:

14 December 2020

Duration:

14 December 2020 (13h30) - 15 December 2020 (13h30)

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General context

The concept of liquid-liquid phase separation provides a rationale for the cellular organization of cytoplasmic and nuclear components into biomolecular condensates. These biomolecular condensates can serve as specialized regions for biochemical reactions, dynamically sequester components or act as organizational hubs for signaling networks. In recent years, the interests in how these biomolecular condensates are formed, how they are organized, and what their functional properties are has grown substantially, and has led to increasing evidence that dysregulation of liquid-liquid phase separation contributes to neurodegenerative diseases as well as cancer. To keep up to date with the latest developments and break-throughs in this field, we organize a VIB training day on ‘Liquid-Liquid Phase Separation’.

Objectives

The aim of this training day is – after a brief introduction – to provide guidelines for accurate experimental characterization of LLPS processes in vitro and in cells. We will end the day with a discussion on the implications of dysregulation of liquid-liquid phase separation for human disease. We have invited top-notch speakers on this topic to inform you on the latest developments.  

Event intended for

This training targets scientists who want to keep up-to-date with the latest evolutions in the field of liquid-liquid phase separation. The training is aimed at PhD students, postdoctoral fellows, technicians or other scientists with a molecular biology background.

Scientific committee

Eleonora Leucci, Department of Oncology, KU Leuven

Thomas Moens, VIB-KU Leuven Center for Brain & Disease Research

Peter Tompa, VIB-VUB Center for Structural Biology

Extra information
  • Participation is free for all participants.
  • Note that upon no-show without valid justification you will be blacklisted for the VIB training program for 1 year. Click here for more information.

Trainers

Steven Boeynaems
Department of Genetics, Stanford University School of Medicine, US
Alex Holehouse
Department of Biochemistry & Molecular Biophysics, Washington University School of Medicine, US
Avinash Patel
Dewpoint Therapeutics, Dresden, DE
Saskia Hutten
BioMedical Center, Cell Biology, Ludwig-Maximilians-University Munich, DE
Ina Vorberg
German Center for Neurodegenerative Diseases Bonn (DZNE), Bonn, DE
Kaveh Daneshvar
Harvard Medical School, Massachusetts General Hospital, US

Program

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Online registration

Introduction

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Welcome

Engineering protein phase separation: decoding/encoding function in natural and synthetic systems
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Steven Boeynaems, Department of Genetics, Stanford University School of Medicine, US 

TBA
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Alex Holehouse, Department of Biochemistry & Molecular Biophysics, Washington University School of Medicine, US

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Q&A - networking break

Function of condensates

Control of phase separation by nuclear import receptors
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Saskia Hutten, BioMedical Center, Cell Biology, Ludwig-Maximilians-University Munich, DE

Control of gene activation by phase-separated lncRNA-protein condensates
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Kaveh Daneshvar, Harvard Medical School, Massachusetts General Hospital, US

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Q&A - Networking

In vitro assays

Biochemical reconstitution of biomolecular condensates to interrogate cell physiology and disease
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Avinash Patel, Dewpoint Therapeutics, Dresden, DE

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TBC

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Q&A - Networking break

Dysregulation of phase separation and its implications

Aggregation and spreading behavior of a prion-like domain in mammalian cells
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Ina Vorberg, German Center for Neurodegenerative Diseases Bonn (DZNE), Bonn, DE

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TBC

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Q&A - Networking - closing