training

VIB Bioimaging Core Spring Brunch

VIB Bioimaging Core Spring Brunch

26 May 2021 - 28 May 2021

Online course

VIB Bioimaging Core Spring Brunch

online
imaging
Location:

Online course

Start date:

26 May 2021

Duration:

26, 27 & 28 May 2021, 10h30 - 14h30 (CET)

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General context

Basic and advanced light microscopy training. 

With the growing impact of state-of-the-art microscopy techniques in life sciences, it is the right time to upgrade your imaging skills and benefit from it in your future research. This training event focuses on light microscopy techniques available at the the VIB BioImaing Core and covers basic and advanced applications. 

Objectives

On the first day, we will cover the fundamentals of optical microscopy. In the second session, we will dive into more advanced microscopy techniques and functional applications. Also, a discussion on imaging ethics will be organized. The topic of the final session is the basic concepts of image processing. Please, check out the program for more details. 

All trainers are microscopy experts from the VIB BioImaging Core, who are happy to support you also beyond this training event.

Approach

During this training, we offer lectures on principles and theory behind several light microscopy techniques, and Q&A and discussion rounds where we welcome you to address any microscopy related questions you may have for your scientific project. Please note that the different lectures are inter-connected and that upon registration to this training, you are committed to attending the 3 entire brunch sessions.

Event intended for

This training targets scientists who want to learn about basic and advanced light microscopy applications. It is aimed at Ph.D. students, postdoctoral fellows, technicians or other scientists life sciences who require microscopy for their research.

Scientific committee

Saskia Lippens, VIB Bioimaging Core, Ghent

Sebastian Munck, VIB Bioimaging Core, Leuven

Course materials
Extra information
  • Participation is free for academics and researchers from research institutes.
  • Note that upon no-show without valid justification you will be blacklisted for the VIB training program for 1 year.

Trainers

Saskia Lippens
VIB Bioimaging Core, Ghent, BE
Sebastian Munck
VIB Bioimaging Core, Leuven, BE
Nikky Corthout
VIB-KU Leuven Center for Brain & Disease Research, BE
Amanda Gonçalves
VIB Bioimaging Core Ghent, BE
Eef Parthoens
VIB Bioimaging Core Ghent, BE
Evelien Van Hamme
VIB Bioimaging Core Ghent, BE
Axelle Kerstens
VIB Bioimaging Core, Leuven, BE
Abril Escamilla Ayala
VIB Bioimaging Core Leuven, BE
Gert Van Isterdael
VIB Flow Core, Ghent, BE
Dominique Audenaert
VIB Screening Core, Ghent, BE

Program

26 May 2021 (10h30 - 14h30):

  • Basics of optical microscopy (magnification, resolution, diffraction, lenses, fluo basic, Slide scanner
  • The point scanning and spinning disk confocal
  • Imaging of living samples 
  • Tools for fluorescent imaging 
  • Immunolabeling techniques 
  • General Q&A 
  • Live Demo and time for immediate questions 

27 May 2021 (10h30 - 14h30):

  • An overview of the VIB BioImaging Core portfolio 
  • F-techniques: FRAP, FRET, FLIM 
  • Breaking the resolution limit with deconvolution-based methods
  • Super-resolution techniques: SIM, PALM/STORM and STED, TIRF 
  • Imaging of large samples 
  • Imaging beyond BIC @VIB: VIB Flow Core and VIB Screening Core 
  • Q&A on microscopy projects 
  • Discussion on image ethics

28 May 2021 (10h30 - 12h):

  • Basic concepts of the digital image 
  • Any remaining questions