VIB Bioimaging Core Spring Brunch

VIB Bioimaging Core Spring Brunch

02 May 2022 - 20 May 2022

Online video material + 3 online Q&A sessions

online
imaging
live training

VIB Bioimaging Core Spring Brunch

Target Audience:
All VIB staff
Location:

Online video material + 3 online Q&A sessions

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General context

Basic and advanced light microscopy training. 

With the growing impact of state-of-the-art microscopy techniques in life sciences, it is the right time to upgrade your imaging skills and benefit from it in your future research. This training event focuses on light microscopy techniques available at the the VIB BioImaing Core and covers basic and advanced applications. 

Learning outcomes

In the first week, we will cover the fundamentals of optical microscopy. In the second week, we will dive into more advanced microscopy techniques and functional applications. Also, a discussion on imaging ethics will be organized. The topic of the final and third week is the basic concepts of image processing. Please, check out the program for more details. On every Friday, you will get the chance to ask your questions and learn from the questions of other participants during an interactive Q&A session with the trainers.

All trainers are microscopy experts from the VIB BioImaging Core, who are happy to support you also beyond this training event.

Approach

During this training, we offer online lectures on principles and theory behind several light microscopy techniques that you can follow at your own pace. These lectures are supported by 3 Q&A and discussion rounds where we welcome you to address any microscopy related questions you may have for your scientific project. Please note that the different lectures are inter-connected, so we advice you to watch the lectures in the order suggested in the program. Upon registration to this training, you are committed to attending the 3 Q&A sessions.

Event intended for

This training targets scientists who want to learn about basic and advanced light microscopy applications. It is aimed at Ph.D. students, postdoctoral fellows, technicians or other scientists life sciences who require microscopy for their research.

Scientific committee

Saskia Lippens, VIB Bioimaging Core, Ghent

Sebastian Munck, VIB Bioimaging Core, Leuven

Extra information
  • This training is free for VIB participants.
  • Note that upon no-show without valid justification you will be blacklisted for the VIB training program for 1 year.

Trainers

Sebastian Munck
VIB Bioimaging Core, Leuven, BE
Nikky Corthout
Training & Conferences - Trainer picture - Nikky Corthout
VIB-KU Leuven Center for Brain & Disease Research, BE
Amanda Gonçalves
VIB Bioimaging Core Ghent, BE

Amanda is a Microscopy Expert at the VIB Bioimaging Core-Gent, where since 2011, she has gained expertise in large sample imaging and quantification, from slide scanner to light sheet. She also has over 15 years of experience teaching science, both practical and theoretical, on the graduate and post graduate levels.

Eef Parthoens
Training & Conferences - Trainer picture - Eef Parthoens
VIB Bioimaging Core Ghent, BE
Evelien Van Hamme
VIB Training & Conferences - Trainer picture - Evelien Van Hamme
VIB Spatial Catalyst, BE

Evelien Van Hamme leads the Spatial Catalyst team in VIB. She obtained her PhD in 2009 at the university of Ghent studying viral entry of coronaviruses in monocytes. She has 12 years of experience in advanced bio imaging applications such as live cell imaging, functional imaging and CLEM workflows. In 2018 her interest in spatial technologies was raised when she started evaluating the Akoya CODEX technology. Since 2023 she focuses on spatial technology applications in life science research together with a team of data analysts.

Contact Evelien Van Hamme :
Axelle Kerstens
Axelle Kerstens
VIB Bioimaging Core, Leuven, BE
Abril Escamilla Ayala
VIB Training & Conferences - Trainer picture - Abril Escamilla Ayala
VIB Bioimaging Core Leuven, BE
Gert Van Isterdael
VIB Flow Core Ghent, BE

As head of the VIB Flow Core,  Gert provides flow cytometry expertise to the users of the core facility by giving technical help to the scientists, maintaining the machines, implicating high quality SOPs as well as providing both experimental setup and data analysis consultations. The Flow Core develops novel automated analysis techniques for multi-parameter flow cytometry data to remove user bias in close collaboration with the on-site Bioinformatics team headed by Prof. Yvan Saeys. 

Contact Gert Van Isterdael :
Dominique Audenaert
VIB Training & Conferences - Speaker picture - Dominique Audenaert
VIB Screening Core Ghent, BE

Program

Week 1 (2 - 6 May 2022):

  • Basics of optical microscopy (magnification, resolution, diffraction, lenses, fluo basic)
  • The point scanning and spinning disk confocal
  • Imaging of living samples 
  • Tools for fluorescent imaging 
  • Immunolabeling techniques 
  • Demo
  • Q&A session: Friday 6 May 2022, 13h - 14h

Week 2 (9 - 13 May 2022):

  • An overview of the VIB BioImaging Core portfolio 
  • F-techniques: FRAP, FRET, FLIM 
  • Breaking the resolution limit with deconvolution-based methods
  • Super-resolution techniques: SIM, PALM/STORM and STED, TIRF 
  • Imaging of large samples 
  • Imaging beyond BIC @VIB: VIB Flow Core and VIB Screening Core 
  • Q&A session: Friday 13 May 2022, 13h - 14h

Week 3 (16 - 20 May 2022):

  • Basic concepts of the digital image 
  • Image ethics
  • Q&A session: Friday 20 May 2022, 13h - 14h

Practical info

Extra information

Participants will receive a link to all the video material on 2 May 2022. Note that the lectures are best watched in a specific order (as indicated in the program). Each week, there will be a Q&A session on a specific set of lectures. The link to attend this online live session will be provided to the participants a few days in advance.