Bulk RNASeq in Galaxy: from reads to counts

This practical aims to familiarize you with the Galaxy user interface & execute a complete analysis of RNA-seq data.

It will teach you how to perform basic tasks such as importing data, running tools, working with histories, creating workflows, and sharing your work. In this tutorial we will introduce you to bare basics of Galaxy:

• Getting data from external databases such as UCSC
• Performing simple data manipulation
• Understanding Galaxy’s History system
• Creating and running a workflow

The RNA-seq data analysis part comprises:

• Filtering and quality control of reads
• Mapping of reads to reference genomes
• Read summarization and read count modes

This workshop is organised by ELIXIR Belgium. The course will handle the data analysis of bulk RNA-seq up till counts data. The subsequent part of the data analysis (differential expression) will be addressed during the course "Bulk RNASeq: from counts to differential expression".