Analyzing flow cytometry data using FlowJo - Leuven
General context
FlowJo version10 is a newly designed version of the famous FlowJo software. In contrast with previous versions (iOS only), version 10 is now a multi-platform software tool (Windows, iOS, Linux). It now has a very intuitive user interface designed to analyze your data in a very practical and fast way.
The training will be focused on getting familiar with all the tools available within the software.
Learn researchers how to analyze flow cytometry data in an efficient way using FlowJo v10,the world’s nr.1 analysis software tool for flow cytometry data.
This training will make you familiar with all the basic and advanced features of FlowJo v10. In the end you will be able to analyze and visualize your flow data and learn how to get the statistics out of it
Prior knowledge in general flow cytometry is required but the general principles will be repeated.
Trainers
Gert Van Isterdael
As head of the VIB Flow Core, Gert provides flow cytometry expertise to the users of the core facility by giving technical help to the scientists, maintaining the machines, implicating high quality SOPs as well as providing both experimental setup and data analysis consultations. The Flow Core develops novel automated analysis techniques for multi-parameter flow cytometry data to remove user bias in close collaboration with the on-site Bioinformatics team headed by Prof. Yvan Saeys.
Program
Flow cytometry, multicolor flow and compensation: general principles
General layout introduction
Workspace overview
Creating, handling and saving workspaces
Creation of groups
Gating procedures
Creating statistics tables with the Table editor
Data visualization with Layout editor
Batch analysis features
Automated compensation
Practical exercise with some datasets
Practical info
18 April 2023
Leuven - Campus Gasthuisberg
Herestraat 49
3000 Leuven
Belgium
18 April 2023
Leuven - Campus Gasthuisberg
Please use Parking West - Follow 'Dagcentrum Onco' to get to the O&N buildings. Take the stairs on you left after exiting Parking West. You are now in front of buidling O&N4 and on the left you find O&N5.