Apply MutationExplorer to study mutation of proteins and visualise their energetic impact
6 March, 9h - 13h
General context
The possible effects of mutations on stability and function of a protein can only be understood in the context of protein 3D structure. The MUTATIONEXPLORER webserver maps variants onto protein structures and allows users to study variation by inputting sequence changes. As the user enters variants, the 3D model evolves, and estimated changes in energy are highlighted. In addition to a basic per-residue input format, MUTATIONEXPLORER can also upload an entire replacement sequence. Previously the purview of desktop applications, such an upload can back-mutate PDB structures to wildtype sequence in a single step. Structures are flexibly colorable, not only by energetic differences, but also by hydrophobicity, or sequence conservation, or other biochemical profiling. Another mutation source can be human single nucelotide polymorphisms (SNPs), genomic coordinates input in VCF format. MUTATIONEXPLORER strives for efficiency in user experience. For example, 45,000 PDB depositions for instant retrieval and initial display have been prepared. All modeling steps are performed by Rosetta.
- quantify the effect of mutations on the 3D structure of proteins
- visualise the impact of mutations on the 3D structure of proteins
MutationExplorer https://proteinformatics.uni-leipzig.de/mutation_explorer/
Trainers
Amelie Stein
My lab studies the consequences of sequence variants on proteins, focusing on their cellular stability and function. We perform high-throughput assays on protein variants and build on this data to develop and improve methods for prediction of variant consequences. We then apply these methods to determine whether genomic variants are likely to be pathogenic. Further we aim to integrate effects of multiple mutations for applications in protein engineering.
Practical info
06 March 2024
Leuven - Provinciehuis Vlaams-Brabant
Provincieplein 1
3010 Leuven
Belgium
06 March 2024
Leuven - Provinciehuis Vlaams-Brabant
Leuven is easy to reach by train from all Belgian and European cities. Leuven is a 20-minute train ride from Brussels, 45 minutes from Antwerp, 50 minutes from Liège, 1.5 hours from Paris and 2.5 hours from London, Amsterdam or Cologne.
06 March 2024
Leuven - Provinciehuis Vlaams-Brabant
Leuven is located at the junction of two important highways: the E40 and the E314. The 'P' signposts will guide you to the different car parks. If you are using a GPS, please enter “Tiensevest” and not “Provincieplein 1”.
06 March 2024
Leuven - Provinciehuis Vlaams-Brabant
+32 16 26 70 15
provinciehuis@vlaamsbrabant.be