Introduction to the analysis of NGS data, online

online
omics
ELIXIR
live training

Introduction to the analysis of NGS data, online

Target Audience:
VIB PhD Student
VIB Postdoc
VIB Staff Scientist
VIB Group leader & Expert
VIB Technical support
Location:

online

Duration:

2 days

General context

This course combines e-learning with live online sessions.
________________________________________________________________________________________________________________

This training is an introduction to 'Bulk RNASeq analysis for differential expression', and 'Hands on introduction to ChIPSeq analysis'. If you want to follow one of these trainings and you have no experience with NGS data you should follow this introduction first. The course will not prepare you for the single cell trainings. 

Since part of the course is done via e-learning you have to take into account that you will have to spend time to this course outside of class.

Objectives

This training is an introduction to a series of trainings on the analysis of Next Generation Sequencing data. This training is intended for newbies to the field and will teach them all background knowledge required to successfully complete the advanced NGS analysis trainings. To this end the training will give an overview of:
- The Illumina platform
- NGS data formats and file handling
- Quality control of NGS data
- Mapping of NGS data
- Supporting IT: Galaxy platform

Required skills

None

Software demonstrated

- Galaxy: http://usegalaxy.be

- FastQC

- Trimmomatic

- Bowtie/BWA

- STAR/HISAT2

- Kallisto/Salmon

- Picard

- Qualimap

- RSeQC

- IGV

Extra information

   

Trainers

Program

9h30-12h exercises on checking the quality of the data

13h-16h30 improving the quality of the data

9h30-12h mapping DNASeq data

13h-16h30 mapping RNASeq data

Practical info

Extra information

Info for trainer
Elixir code: 7471