Nextflow for reproducible and automated data analysis

advanced bioinformatics
research data management
programming
ELIXIR
live training

Nextflow for reproducible and automated data analysis

Target Audience:
All VIB staff
Location:

Ghent - VIB/UGent FSVM II

Duration:

16 and 17 November

General context

You will learn how to use Nextflow for building scalable and reproducible bioinformatics pipelines and running them on a personal computer, cluster and cloud. Starting from the basic concepts we will build our own simple pipeline and add new features with every step, all in the new DSL2 language.

Objectives
  • Understand Nextflow's basic concepts: channels, processes, modules, workflows, etc. 
  • Write and run a Nextflow pipeline 
  • Write and modify config files for storing parameters related to computing hardware as well as pipeline dependent parameters
Event intended for

Bioinformaticians with no or little knowledge of containers or workflow pipelines.

Required skills

You're familiar with doing bioinformatics on the command-line and with Docker.

  •  If you have no experience with command line we strongly recommend to follow the Linux initiation training first.
  •  If you have no experience with Docker you can follow the Docker training. 

Trainers

Kobe Lavaerts

Bioinformatician at VIB Nucleomics Core in Leuven. Currently mainly focusing on operating and developing routine pipelines for processing raw sequencing data.

Contact Kobe Lavaerts :
Kristofer Davie

Bioinformatician with over 8 years of experience in the analysis of a wide variety of next generation sequencing data types, with a strong focus on the analysis of single cell data (sc/snRNA-seq, scATAC-seq, Spatial technologies).

Contact Kristofer Davie :

Program

09:30 - 10:30 - Bulding Blocks

10:30 - 10:45 - Break

10:45 - 12:00 - Processes and Workflows

12:00 - 13:00 - Lunch

13:00 - 14:45 - Creating our first pipeline

14:45 - 15:00 - Break

15:00 - 17:30 - Modules and Subworkflow

09:30 - 10:45 - Config files, Containers and Reports

10:45 - 11:00 - Break

11:00 - 12:30 - Exercise

12:30 - 13:30 - Lunch

13:45 - 15:00 - Project Challenge

15:00 - 15:15 - Break

15:15 - 17:00 - Project Challenge

Practical info

Location & Venue

16 November 2023 - 17 November 2023

Ghent - VIB/UGent FSVM II

Technologiepark 75
9052 Zwijnaarde
Belgium

Public transport

16 November 2023 - 17 November 2023

Ghent - VIB/UGent FSVM II
Public transport

From Ghent Sint-Pieters station, you can take bus 49, 50 or 70 to Technologiepark. Please check Routeplanner De Lijn for schedules.

Route description

16 November 2023 - 17 November 2023

Ghent - VIB/UGent FSVM II
Parking

Route description

There is only one entrance to Technologiepark. At the entrance, please take a ticket and have it validated at the reception desk of the FSVM building. Parking will only be allowed in regular parking spots (for instance in front of the building) and in the new parking tower. Parking alongside the roads or in other places where there is no regular parking is prohibited and after initial warnings, fines will be issued (€ 50).

Venue contact

16 November 2023 - 17 November 2023

Ghent - VIB/UGent FSVM II