Recombinant Protein Expression
22 November 2021
General context
This training will guide the attendees through the key points to consider when one wants to produce and purify high quantities of functional proteins such as antibodies for non-clinical experimental use or as biotherapeutics.
The training will start with an introduction on the available recombinant protein expression systems, with their advantages and limitations, from which to choose for your favorite protein. Also, high-throughput protein expression, expression in plant cells, antibody and nanobody expression and a difficult-to-express protein case will be discussed. A next session will focus on strategy design to optimize protein purification and how to address quality assurance of the purified proteins. For clinical use of a protein-based biological stability and production yields are critical issues that come on top of the desired therapeutic efficacy. Why and how to deal with these critical aspects will be addressed as well during the training. Furthermore, also the key steps that are required for optimal protein formulation will be reviewed. We plan to end the day with a keynote talk from a pharma industry speaker illustrating the issues that need to be considered when engineering recombinant proteins for clinical applications.
This training targets scientists who want to learn about the latest developments in recombinant protein expression and purification. It is aimed at PhD students, postdoctoral fellows, technicians or other scientists with a molecular biology background.
Jurgen Haustraete, VIB Protein Core, Ghent
Xavier Saelens, VIB-UGent Center for Medical Biotechnology
Hannes Vanhaeren, VIB-UGent Center for Plant Systems Biology
- Participation is free for VIB participants.
- Registration fee for Academics from Universities and Research Centers is € 50 (21% VAT incl.).
- Registration fee for participants of EU-LIFE partner institutes is € 50 (21% VAT incl.).
- Registration fee for industry is € 300 (21% VAT incl.).
- Note that upon no-show without valid justification you will be blacklisted for the VIB training program for 1 year and for VIB participants the reimbursement of the catering costs (€ 50) will be charged to the research group. Click here for more information.
Program
Welcome Coffee
Introduction
Introduction to recombinant protein expression and overview of the different expression systems
Jurgen Haustraete, VIB Protein Core, Ghent, BE
High-throughput protein expression
Ray Owens, Oxford Protein Production Facility-UK, University of Oxford, UK
Coffee
Protein Expression
Plant-made biopharmaceuticals
Nobuyuki Matoba, Department of Pharmacology and Toxicology, University of Louisville School of Medicine, US
Nanobody expression
Nick Devoogdt, Laboratory for In vivo Cellular and Molecular Imaging, ICMI-BEFY, VUB, BE
Adaption of human antibody λ and κ light chain architectures to CDR repertoires
TBC, Switch lab, VIB-KU Leuven Center for Brain & Disease Research, BE
Lunch
Protein Purification and Quality Assurance
Case study
TBC, Savvides lab, VIB-UGent Center for Inflammation Research, BE
Protein purification
Iman Akhazzan, GE Healthcare, BE
Quality assurance
Kevin Balcaen, VIB Protein Core, Ghent, BE
Coffee
Protein Formulation
Target de-risking
Bruno Dombrecht, VIB Discovery Sciences, BE
GMP and formulation
Jody Voorspoels, Eurofins Belgium, BE
Keynote
Engineering recombinant proteins for clinical applications
TBC
Practical info
22 November 2021
UGent-VIB Research Building FSVM
Technologiepark 71
9052 Zwijnaarde
Belgium
22 November 2021
UGent-VIB Research Building FSVM
At Gent St Pieters station you can take the following city buses: Buses 70, 71 and 72 ("Tramstraat"). You get off at the stop marked "Bollebergen" (on the Grote Steenweg Noord). The Technology Park is straight in front of you on the other side of the soft verge. Walk into the park to the roundabout and then straight on. The UGent-VIB research building is the first building on the left.
There is also a shuttle bus between Gent-Sint-Pieters and Technologiepark, more information can be found here.
22 November 2021
UGent-VIB Research Building FSVM
There is free parking available in front of the building.
22 November 2021
UGent-VIB Research Building FSVM
+32 9 331 36 00