training

Recombinant Protein Expression, postponed

Recombinant Protein Expression, postponed

Location:

Ghent, UGent-VIB Research Building FSVM

Start date:

09 June 2020

Duration:
09 June 2020

General context

Due to Coronavirus spreading in Belgium and Europe, VIB has made the difficult decision to postpone the training on 'Recombinant Protein Expression' until 22 June 2021. We will contact you in due time to register for the event next year.

 

This training will guide the attendees through the key points to consider when one wants to produce and purify high quantities of functional proteins such as antibodies for non-clinical experimental use or as biotherapeutics.

Objectives

The training will start with an introduction on the available recombinant protein expression systems, with their advantages and limitations, from which to choose for your favorite protein. Also, high-throughput protein expression, expression in plant cells, antibody and nanobody expression and a difficult-to-express protein case will be discussed. A next session will focus on strategy design to optimize protein purification and how to address quality assurance of the purified proteins. For clinical use of a protein-based biological stability and production yields are critical issues that come on top of the desired therapeutic efficacy. Why and how to deal with these critical aspects will be addressed as well during the training. Furthermore, also the key steps that are required for optimal protein formulation will be reviewed. We plan to end the day with a keynote talk from a pharma industry speaker illustrating the issues that need to be considered when engineering recombinant proteins for clinical applications.

Event intended for

This training targets scientists who want to learn about the latest developments in recombinant protein expression and purification. It is aimed at PhD students, postdoctoral fellows, technicians or other scientists with a molecular biology background.

Scientific committee

Jurgen Haustraete, VIB Protein Core, Ghent

Xavier Saelens, VIB-UGent Center for Medical Biotechnology

Hannes Vanhaeren, VIB-UGent Center for Plant Systems Biology

Extra information
  • Participation is free for VIB participants.
  • Registration fee for Academics from Universities and Research Centers is € 50 (21% VAT incl.).
  • Registration fee for participants of EU-LIFE partner institutes is € 50 (21% VAT incl.).
  • Registration fee for industry is € 300 (21% VAT incl.).
  • Note that upon no-show without valid justification you will be blacklisted for the VIB training program for 1 year and for VIB participants the reimbursement of the catering costs (€ 50) will be charged to the research group. Click here for more information.

Program

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Welcome Coffee

Introduction

Introduction to recombinant protein expression and overview of the different expression systems
-

Jurgen Haustraete, VIB Protein Core, Ghent, BE

High-throughput protein expression
-

Ray Owens, Oxford Protein Production Facility-UK, University of Oxford, UK

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Coffee

Protein Expression

Plant-made biopharmaceuticals
-

Nobuyuki Matoba, Department of Pharmacology and Toxicology, University of Louisville School of Medicine, US

Nanobody expression
-

Nick Devoogdt, Laboratory for In vivo Cellular and Molecular Imaging, ICMI-BEFY, VUB, BE

Adaption of human antibody λ and κ light chain architectures to CDR repertoires
-

TBC, Switch lab, VIB-KU Leuven Center for Brain & Disease Research, BE

-

Lunch

Protein Purification and Quality Assurance

Case study
-

TBC, Savvides lab, VIB-UGent Center for Inflammation Research, BE

Protein purification
-

Iman Akhazzan, GE Healthcare, BE

Quality assurance
-

Kevin Balcaen, VIB Protein Core, Ghent, BE

-

Coffee

Protein Formulation

Target de-risking
-

Bruno Dombrecht, VIB Discovery Sciences, BE

GMP and formulation
-

Jody Voorspoels, Eurofins Belgium, BE

Keynote

Engineering recombinant proteins for clinical applications
-

TBC

Practical info

Location & Venue

09 June 2020

UGent-VIB Research Building FSVM

Technologiepark 71
9052 Zwijnaarde
Belgium

Public transport

09 June 2020

UGent-VIB Research Building FSVM
Public transport

Itineraries:

At Gent St Pieters station you can take the following city buses: Buses 70, 71 and 72 ("Tramstraat"). You get off at the stop marked "Bollebergen" (on the Grote Steenweg Noord). The Technology Park is straight in front of you on the other side of the soft verge. Walk into the park to the roundabout and then straight on. The UGent-VIB research building is the first building on the left.

There is also a shuttle bus between Gent-Sint-Pieters and Technologiepark, more information can be found here.

 

Route description

09 June 2020

UGent-VIB Research Building FSVM
Parking

Route description

There is free parking available in front of the building.

Venue contact

09 June 2020

UGent-VIB Research Building FSVM
Location contact

+32 9 331 36 00