Summer School in Advanced Light Microscopy
Summer School in Advanced Light Microscopy
General context
The 6th VIB summer school in advanced light microscopy is an opportunity for Ph.D. students, post-Doctoral fellows, staff scientists and technical staff to receive training in the skills needed to image the micro-world using the latest in advanced microscopy techniques and equipment. The course begins with the basic concepts and theory of optical microscopy and proceeds using a combination of lectures and practical exercises through advanced light microscopy including live cell and tissue imaging, functional microscopy (FRAP/FRET/FLIM), advanced fluorescence and super-resolution microscopy.
This course is intended for those with limited or no prior training or those who wish to refresh their skills base.
Saskia Lippens, VIB BioImaging Core Ghent, BE
Sebastian Munck, VIB Bioimaging Core Leuven, BE
- The training fee for VIB and other academic participants is 390 euro (incl. VAT).
- The training fee for industry is 1300 euro (incl. VAT).
- The fee includes access to the training and all training materials, coffee breaks and lunches for the four days. On Monday and Wednesday, there is a dinner foreseen. You can subscribe for the dinners during registration. There is no extra fee charged for attending the dinners.
- The fee does not include travel or accomodation.
- Note that upon no show without valid justification you will not be refunded and you will be blacklisted for the VIB training program for 1 year. Click here for more information
Trainers
Sebastian Munck
Chris Guérin
Peter O' Toole
Winnok De Vos
Karin Aumayr
Kurt Anderson
Daniël Van Damme
Ricardo Henriques
Stephanie Reichelt
Natalia Gunko
Benjamin Pavie
Bob Asselbergh
Bob is staff scientist at the VIB Center for Molecular Neurology’s Bio-Imaging Expertise Unit, with an interest in quantitative fluorescence assays, automated high throughput microscopy and superresolution expansion microscopy.
Evelien Mylle
Eef Parthoens
Nikky Corthout
Amanda Gonçalves
Amanda is a Microscopy Expert at the VIB Bioimaging Core-Gent, where since 2011, she has gained expertise in large sample imaging and quantification, from slide scanner to light sheet. She also has over 15 years of experience teaching science, both practical and theoretical, on the graduate and post graduate levels.
Evelien Van Hamme
Evelien Van Hamme leads the Spatial Catalyst team in VIB. She obtained her PhD in 2009 at the university of Ghent studying viral entry of coronaviruses in monocytes. She has 12 years of experience in advanced bio imaging applications such as live cell imaging, functional imaging and CLEM workflows. In 2018 her interest in spatial technologies was raised when she started evaluating the Akoya CODEX technology. Since 2023 she focuses on spatial technology applications in life science research together with a team of data analysts.
Program
Light Microscopy Basics
Registration, Lobby
Introduction, seminar room
Lecture 1 – The basics of light microscopy: History, Basic optics, diffraction, lenses, optical aberrations and Köhler illumination
Chris Guérin, seminar room
Practical 1 – Setting up Köhler illumination
Small Cafetaria
Lunch, lobby
Lecture 2 – Contrast and imaging methods, phase contrast (PC) and differential interference contrast (DIC)
Sebastian Munck, seminar room
Practical 2 – Setting up PC and how to keep a microscope clean
Small Cafetaria
Lecture 3 – Fluorescence, fluorescence microscopy and immunohistochemistry
Saskia Lippens, seminar room
Coffee break & Practical 3 – Choosing filters for fluorescence imaging
Lobby/cafetaria
Lecture 4 – Basics of confocal imaging, Z-sectioning, spinning disk.
Peter O'Toole, seminar room
Lecture 5 – 2-photon imaging, live cell imaging
Winnok De Vos, seminar room
Dinner
Advanced and functional light microscopy
Lecture 6 – Making the unseen appear spectral unmixing, deconvolution, structured illumination
Karin Aumayr, seminar room
Lecture 7 – Functional microscopy, FRET/FLIM
Kurt Anderson, seminar room
Coffee break, lobby
Practical 4 – Intro to confocal imaging
Lunch, lobby
Lecture 8 – Fluorescence confocal imaging of plants
Daniël Van Damme, seminar room
Lecture 9 – Fluorescence Nanoscopy
Ricardo Henriques, seminar room
Coffee break, lobby
Lecture 10 - Fluorescent probes and delivery strategies
Sebastian Munck, seminar room
Lecture 11 – Correlative light and electron microscopy
Natalia Gunko, seminar room
Lecture 12 – Overview on image processing methods
Benjamin Pavie, seminar room
Advanced Microscopy - Confocal rotations
Confocal microscopy - rotation 1
Coffee break, lobby
Confocal Microscopy - rotation 2
Lunch, lobby
Confocal Microscopy - rotation 3
Coffee break, lobby
Confocal Microscopy - rotation 4
Apero & BBQ
Advanced Microscopy - practicals (2)
Confocal Microscopy - rotation 5
Coffee break, atrium
Confocal microscopy - rotation 6
Task on fraude in image processing and Lunch, atrium
Lecture 13: Imaging large samples
Stephanie Reichelt, seminar room
Practical - The code of conduct what is allowed in image processing for publishing
Sebastian Munck & Saskia Lippens, seminar room
Wrap up
Practical info
Ghent - VIB/UGent FSVM I
Technologiepark 71
9052 Zwijnaarde
Belgium
Ghent - VIB/UGent FSVM I
From Ghent Sint-Pieters station, you can take a bus to Technologiepark. Please check Routeplanner De Lijn for schedules.
Ghent - VIB/UGent FSVM I
Looking for a custom cycling route? Try the online cycling route planner to easily and quickly identify the fastest, safest and/or most scenic route to any destination in Ghent.
Cycling route planner
Shared bicycles
Several providers are active in the area.
Take a look at their platforms:
- Indigo Weel Pro app
- Bluebike
- DOTT
- BOLT eBikes
- Donkey Republic
There are also some public bicycle pumps at the park.
Ghent - VIB/UGent FSVM I
There is only one entrance to Technologiepark. At the entrance, please take a ticket - Parking is only allowed in regular parking spots (for instance in front of the building) and in the parking tower. Parking alongside the roads or in other places where there is no regular parking is prohibited.
Ghent - VIB/UGent FSVM I
+32 9 331 36 00