Analyzing qPCR data with qbase+

Analyzing qPCR data with qbase+

General context

This hands-on training provides an introduction to the analysis of qPCR data using qbase+. Although this software allows to do various types of analyses (gene expression, copy number analysis, miRNA profiling), we will focus in the training on gene expression analysis.


This training specifically focuses on the analysis of gene expression data using qbase+. During this training you will:

  • create an experiment in qbase+
  • import run data
  • customize sample properties
  • select reference genes
  • assess the quality of your qPCR data
  • visualize and export the results
Required skills

Qbase+ is freely distributed by BITS to VIB scientists. Therefore the training will be limited to VIB scientists.

The training is intended for people with basic knowledge of the principles underlying qPCR.


Christof De Bo
Staff employee Research Informatics at VIB
Contact Christof De Bo :



designing primers for qPCR​​​​​ + exercises


qPCR experiment design + exercises


qbase+ demo part I


qbase+ demo part II


qbase+ exercises and questions

Practical info

Clemenspoort Gent

Overwale 3
9000 Ghent

Clemenspoort Gent
Public transport

The train station Ghent St-Pieters is only 250 m away. We have 40 bike lots.

Clemenspoort Gent

We offer parking lots (1.20 € per hour). GPS address: Sint-Denijslaan 251.
Please take the neem the exit SPORTHAL HoGent (not Campus HoGent) and drive all the way to the end, so past the parking slots of HoGent.

Clemenspoort Gent
Location contact

+32 476 46 10 40


VIB training
Tel: +32 9 244 66 11