training

Recombinant Protein Expression

Recombinant Protein Expression

22 November 2021

3Square, Ghent

live training

Recombinant Protein Expression

Target Audience:
All VIB staff
Location:

3Square, Ghent

General context

This training will guide the attendees through the key points to consider when one wants to produce and purify high quantities of functional proteins such as antibodies for non-clinical experimental use or as biotherapeutics.

Objectives

The training will start with an introduction on the available recombinant protein expression systems, with their advantages and limitations, from which to choose for your favorite protein. Also, high-throughput protein expression, expression in plant cells, antibody and nanobody expression and a difficult-to-express protein case will be discussed. A next session will focus on strategy design to optimize protein purification and how to address quality assurance of the purified proteins. For clinical use of a protein-based biological stability and production yields are critical issues that come on top of the desired therapeutic efficacy. Why and how to deal with these critical aspects will be addressed as well during the training. Furthermore, also the key steps that are required for optimal protein formulation will be reviewed. 

Event intended for

This training targets scientists who want to learn about the latest developments in recombinant protein expression and purification. It is aimed at PhD students, postdoctoral fellows, technicians or other scientists with a molecular biology background.

Scientific committee

Jurgen Haustraete, VIB Protein Core, Ghent

Xavier Saelens, VIB-UGent Center for Medical Biotechnology

Hannes Vanhaeren, VIB-UGent Center for Plant Systems Biology

Extra information
  • Participation is free for VIB participants.
  • Registration fee for Academics from Universities and Research Centers is € 50 (21% VAT incl.).
  • Registration fee for participants of EU-LIFE partner institutes is € 50 (21% VAT incl.).
  • Registration fee for industry is € 300 (21% VAT incl.).
  • Note that upon no-show without valid justification you will be blacklisted for the VIB training program for 1 year and for VIB participants the reimbursement of the catering costs (€ 50) will be charged to the research group. Click here for more information.
  • Specific COVID-19 measures: VIB Training follows the rules of the Belgian Government. This implies that to participate in this training, we will scan your Covid Safe Ticket at the reception desk. More information can be found here.

Trainers

Jurgen Haustraete
VIB Protein Core, UGent, BE
Ray Owens
Oxford Protein Production Facility-UK, University of Oxford, UK
Stefan Schillberg
Fraunhofer Institute for Molecular Biology and Applied Ecology IME, DE
Steve Schoonooghe

VIB Nanobody Core, VUB, BE

Julie Andries
Savvas Savvides lab, VIB-UGent Center for Inflammation Research, BE
Joost Haelewyn
Cytiva, BE
Kevin Balcaen
VIB Protein Core, Ghent, BE
Bruno Dombrecht
VIB Discovery Sciences, BE
Hristo Svilenov
Faculty of Farmaceutical Sciences, UGent, BE
Fons Bosman
Eurofins Belgium, BE

Program

-

Welcome Coffee

Introduction

Introduction to recombinant protein expression, production, purification and quality control
-

Jurgen Haustraete, VIB Protein Core, UGent, BE

Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins
-

Ray Owens, Oxford Protein Production Facility-UK, University of Oxford, UK

-

Coffee

Protein Expression

Production of recombinant proteins in plantsystems
-

Stefan Schillberg, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, DE

Nanobody expression strategies - One for each occasion
-

Steve Schoonooghe, VIB Nanobody Core, VUB, BE

-

Lunch

Protein Purification and Quality Assurance

The ups and downs of a chromatogram
-

Julie Andries, Savvides lab, VIB-UGent Center for Inflammation Research, BE

Protein purification in research and process development
-

Joost Haelewyn, Cytiva, BE

Quality Assurance at the VIB Protein Core
-

Kevin Balcaen, VIB Protein Core, Ghent, BE

-

Coffee

Protein Formulation

Compromis à la belge: taking the right? biologic to the clinic
-

Bruno Dombrecht, VIB Discovery Sciences, BE

Towards rational development of antibody co-formulations
-

Hristo Svilenov, Faculty of Farmaceutical Sciences, UGent, BE 

Development of biologicals at CDMO: R&D with GMP mind-setting
-

Fons Bosman, Eurofins Belgium, BE

-

Closing

Practical info

Location & Venue

22 November 2021

Ghent - 3Square

Rijvisschestraat 124
9052 Ghent
Belgium

Public transport

22 November 2021

Ghent - 3Square
Public transport

No public transport information has been provided for this location.

Route description

22 November 2021

Ghent - 3Square
Parking

There is parking available under the building.

Venue contact

22 November 2021

Ghent - 3Square
Location contact

info@3square.be

09/248 10 00