Recombinant Protein Expression
Recombinant Protein Expression
General context
This training will guide the attendees through the key points to consider when one wants to produce and purify high quantities of functional proteins such as antibodies for non-clinical experimental use or as biotherapeutics.
The training will start with an introduction on the available recombinant protein expression systems, with their advantages and limitations, from which to choose for your favorite protein. Also, high-throughput protein expression, expression in plant cells, antibody and nanobody expression and a difficult-to-express protein case will be discussed. A next session will focus on strategy design to optimize protein purification and how to address quality assurance of the purified proteins. For clinical use of a protein-based biological stability and production yields are critical issues that come on top of the desired therapeutic efficacy. Why and how to deal with these critical aspects will be addressed as well during the training. Furthermore, also the key steps that are required for optimal protein formulation will be reviewed.
This training targets scientists who want to learn about the latest developments in recombinant protein expression and purification. It is aimed at PhD students, postdoctoral fellows, technicians or other scientists with a molecular biology background.
Jurgen Haustraete, VIB Protein Core, Ghent
Xavier Saelens, VIB-UGent Center for Medical Biotechnology
Hannes Vanhaeren, VIB-UGent Center for Plant Systems Biology
- Participation is free for VIB participants.
- Registration fee for Academics from Universities and Research Centers is € 50 (21% VAT incl.).
- Registration fee for participants of EU-LIFE partner institutes is € 50 (21% VAT incl.).
- Registration fee for industry is € 300 (21% VAT incl.).
- Note that upon no-show without valid justification you will be blacklisted for the VIB training program for 1 year and for VIB participants the reimbursement of the catering costs (€ 50) will be charged to the research group. Click here for more information.
- Specific COVID-19 measures: VIB Training follows the rules of the Belgian Government. This implies that to participate in this training, we will scan your Covid Safe Ticket at the reception desk. More information can be found here.
Trainers
Jurgen Haustraete
Ray Owens
Stefan Schillberg
Steve Schoonooghe
VIB Nanobody Core, VUB, BE
Julie Andries
Joost Haelewyn
Kevin Balcaen
Bruno Dombrecht
Hristo Svilenov
Fons Bosman
Program
Welcome Coffee
Introduction
Introduction to recombinant protein expression, production, purification and quality control
Jurgen Haustraete, VIB Protein Core, UGent, BE
Overview of a High-Throughput Pipeline for Streamlining the Production of Recombinant Proteins
Ray Owens, Oxford Protein Production Facility-UK, University of Oxford, UK
Coffee
Protein Expression
Production of recombinant proteins in plantsystems
Stefan Schillberg, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, DE
Nanobody expression strategies - One for each occasion
Steve Schoonooghe, VIB Nanobody Core, VUB, BE
Lunch
Protein Purification and Quality Assurance
The ups and downs of a chromatogram
Julie Andries, Savvides lab, VIB-UGent Center for Inflammation Research, BE
Protein purification in research and process development
Joost Haelewyn, Cytiva, BE
Quality Assurance at the VIB Protein Core
Kevin Balcaen, VIB Protein Core, Ghent, BE
Coffee
Protein Formulation
Compromis à la belge: taking the right? biologic to the clinic
Bruno Dombrecht, VIB Discovery Sciences, BE
Towards rational development of antibody co-formulations
Hristo Svilenov, Faculty of Farmaceutical Sciences, UGent, BE
Development of biologicals at CDMO: R&D with GMP mind-setting
Fons Bosman, Eurofins Belgium, BE
Closing
Practical info
22 November 2021
Ghent - 3Square
Rijvisschestraat 124
9052 Ghent
Belgium
22 November 2021
Ghent - 3Square
No public transport information has been provided for this location.
22 November 2021
Ghent - 3Square
There is parking available under the building.
22 November 2021
Ghent - 3Square
info@3square.be
09/248 10 00